How long does reconstituted peptide last?
Not long. Manufacturers' guidance gives up to about a week in the fridge (2–8 °C) and a few weeks frozen in single-use portions. Dry, sealed powder keeps for years; a solution does not.
Storage
Keep it refrigerated (2–8 °C), protected from light, and avoid repeated freeze-thaw cycles. Bacteriostatic water (with its preservative) gives a longer usable window than plain sterile water.
What manufacturers' stability guidance says
- Sigma-Aldrich (Merck): peptide solutions are generally stable for up to a week at 4 °C. Storage at −20 °C or colder is best.
- Bachem: frozen solutions may be kept for a few weeks. Long-term storage in solution is not recommended, especially for peptides containing asparagine, glutamine, cysteine, methionine or tryptophan.
- GenScript: the shelf life of a peptide in solution is very limited, much shorter than that of the dry powder, and freeze-thaw cycles degrade it.
Bacteriostatic water contains a preservative that limits microbial growth. It protects the solution against contamination, not the peptide against chemical breakdown. Label every portion with the compound, the concentration and the date.
Sources: Sigma-Aldrich (Merck) · Bachem · GenScript
When to discard
Discard if the solution turns cloudy, changes colour or shows particles. Sealed, dry lyophilized powder lasts far longer — often 2+ years frozen.
Frequently asked questions
Can I freeze reconstituted peptide?
Yes, in single-use portions at −20 °C or colder: manufacturers give a few weeks for frozen solutions. Thaw each portion once, because repeated freeze-thaw degrades peptides.